Figure 4.

Rapamycin prevents abnormal growth of Tsc2 KO uteri. Four-week-old WT, Het, and uterine-specific Tsc2 KO mice were injected with rapamycin (Rapa, 5 mg/kg ip, 3 d/wk for 8 wk). (A) At 12 weeks of age, rapamycin prevented uterine overgrowth of KO mice as seen by size (upper panel), morphology (lower panel), and (B) weight. Photos are representative from 3 experiments with identical results. Results portray the mean ± SEM of 3 mice per group per treatment. *, P < .05 relative to untreated uterine-specific Tsc2 KO by Student t test. (C) To confirm inhibition of mTORC1, levels of uterine Tsc2, phosphorylated S6, total S6, and GAPDH were measured by immunoblot.