Figure 6.

Letrozole prevents abnormal growth of Tsc2 KO uteri. Four-week-old WT and uterine-specific Tsc2 KO mice were injected with letrozole (10 μg/mouse daily) sc for 8 weeks. Uterine gross morphology (A) and weights (B) in 12-week-old KO treated with letrozole are similar to those of WT. (C) LH levels in serum were analyzed by sandwich immunoradiometric assay. Results portray the mean ± SEM of 3 mice per treatment. *, P < .05 relative to untreated uterine-specific Tsc2 KO by Student t test. (D) Levels of phosphorylated and total S6 were examined by immunoblot, with 3 representative samples from letrozole-treated WT and KO mice shown on the left. Quantification by densitometry of phosphorylated S6/total S6 for WT and KO mice both treated and not treated with letrozole is shown on the right. The y-axis is pS6/tS6 of letrozole-treated vs untreated uteri for WT and KO mice. *, P < .05 relative to untreated WT by Student t test, n ≥ 3 for each condition.