Skip to main content
. 2013 Aug 14;9(8):766–777. doi: 10.7150/ijbs.5711

Fig 5.

Fig 5

Combination treatment with the autophagy inhibitor 3-MA enhances B19-induced apoptosis. A: HO8910 cells were treated with B19 (10 μM) and/or 3-MA (10 mM) for 12 h. Cell viability was determined using MTT assay. The data are presented as the mean ± SD, n = 5. **P < 0.01 vs. control; #P < 0.05 vs. B19 group. B: HO8910 cells were treated with B19 and/or 3-MA for 12 h and then stained with PI and Annexin V-FITC. Positively stained cells were counted using FACScan. The data are presented as the mean ± SD, n = 3. **P < 0.01 vs. control; #P < 0.05 vs. B19 group. C: Western blot analysis for the expression of GRP78, CHOP and PDI in cells that were treated with B19 and/or 3-MA for 12 h. D, E and F: The quantitation of GRP78, CHOP and PDI protein levels. The data are presented as the mean ± SD, n =3. *P < 0.01 vs. control group, #P < 0.05 vs. B19 group.