Table 2.

Stability of reference gene expression under biotic conditions

Biotic Condition	Reference Gene	geNorm		Normfider		BestKeeper		ΔCt
		Stability	Rank	Stability	Rank			Stability	Rank
Developmental stage	18S	1.02	7	1.15	8	0.85	7	1.32	8
	ACTB	0.78	5	0.80	5	0.92	8	1.08	6
	EF1	0.37	1	0.47	3	0.62	5	0.85	3
	GAPDH	0.92	6	1.14	7	0.75	6	1.31	7
	RPL32	0.66	4	0.82	6	0.55	3	1.06	5
	RPS13	0.56	3	0.29	1	0.33	1	0.81	1
	RPS20	0.46	2	0.48	4	0.48	2	0.87	4
	RPS23	0.37	1	0.36	2	0.62	4	0.83	2
Tissue	18S	0.81	3	0.73	4	0.78	7	1.06	4
	ACTB	1.08	7	1.29	8	0.96	8	1.44	8
	EF1	0.86	4	0.40	1	0.34	1	0.92	1
	GAPDH	0.96	6	0.83	6	0.61	4	1.13	6
	RPL32	0.54	1	0.51	2	0.43	2	0.94	2
	RPS13	0.91	5	0.87	7	0.73	6	1.15	7
	RPS20	0.71	2	0.78	5	0.71	5	1.06	5
	RPS23	0.54	1	0.59	3	0.60	3	0.96	3
Strain	18S	0.71	7	0.90	7	0.86	8	1.01	7
	ACTB	0.11	1	0.06	1	0.25	2	0.50	1
	EF1	0.11	1	0.06	1	0.22	1	0.50	1
	GAPDH	0.18	2	0.22	2	0.28	3	0.55	2
	RPL32	0.20	3	0.29	3	0.38	4	0.56	3
	RPS13	0.34	4	0.60	4	0.70	7	0.76	4
	RPS20	0.61	6	0.82	6	0.41	6	0.94	6
	RPS23	0.50	5	0.74	5	0.40	5	0.87	5