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. 2013 Aug 20;9(8):803–810. doi: 10.7150/ijbs.7136

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 3 — CD14 deficiency results in a marginal reduction of B. burgdorferi phagocytosis. (A) RAW264.7 cells were treated with 1 unit of PI-PLC for 20 min at 4 ºC, followed by incubation with Bb914 at an m.o.i = 25. The top panel represents the remaining CD14 surface expression after PI-PLC treatment (red histogram) compared to untreated cells (black histogram). The grey histogram corresponds to the staining with an isotype control antibody. The bottom panel represents internalization of B. burgdorferi. The grey histogram corresponds to a 4 ºC control. (B) CD14-deficient (red histograms) or wild type control BMMs (Black histograms) were assessed by flow cytometry for their capacity to phagocytose Bb914 at m.o.i. = 10 (top panel) and 25 (bottom panel). The grey histogram represents the 4 ºC control. The experiments are representative of at least 3 independent experiments.