Table 2.

Oligonucleotides used in the real-time RT-PCR assays

Assay	Primer	Sequence (5′–3′)	Working concentration (nmol/L)	References
Norovirus GI	QNIF4	CGC TGG ATG CGN TTC CAT	900	da Silva et al. (2007)
	NV1LCR	CCT TAG ACG CCA TCA TCA TTT AC	900	Svraka et al. (2007)
	NVGG1p	(FAM)-TGG ACA GGA GAY CGC RAT CT-(TAMRA)	250	Svraka et al. (2007)
Norovirus GII and MS2 (duplex)	QNIF2	ATG TTC AGR TGG ATG AGR TTC TCW GA	900	Loisy et al. (2005)
	COG2R	TCG ACG CCA TCT TCA TTC ACA	900	Kageyama et al. (2003)
	QNIFS	(FAM)-AGC ACG TGG GAG GGC GAT CG-(TAMRA)	250	Loisy et al. (2005)
	MS2-TM2-F	GGC TGC TCG CGG ATA CCC	200	Dreier et al. (2005)
	MS2-TM2-R	TGA GGG AAT GTG GGA ACC G	700	Dreier et al. (2005)
	MS2-TM2	(JOE)-ACC TCG GGT TTC CGT CTT GCT CGT-(BBQ)	200	Dreier et al. (2005)

Y = C/T; R = A/G; W = A/T