Figure 1.

Moderate throughput screening of miRNAs in cell-based assays. Cells are transfected with individual miRNAs from a miRNAome library in 384-well or other multiwell format (1). Following culture, either image-based (shown) or plate-reader acquisition of data, and subsequent analysis (2), profiles miRNAs by activity shown in a volcano plot (3), providing a dataset for network analysis (4) and Figure 2.