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. 2013 Aug 27;4:223. doi: 10.3389/fphys.2013.00223

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Copyright © 2013 Lemons, Maurya, Subramaniam and Mercola.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Computational and experimental strategies to identify miRNA targets. miRNAs target multiple proteins, and in certain instances a single family of miRNAs target multiple proteins involved in a common biological process, through imprecise basepairing with recognition sequences in mRNA (see text). Commonly used computational and biochemical approaches to identify targets are summarized along with focused strategies for confirming direct interaction of a miRNA with particular mRNA targets.