Fig. 44.

In vivo: in vitro correlation of gene expression alterations (from Heise et al. 2012). Aflatoxin B₁ (AB1), 2-nitrofluorene (2-NF), methapyrilene (MP) and piperonyl-butoxide (PBO) were tested after oral administration to male Wistar rats (gene expression analysis 24 h after administration) and in sandwich cultures of hepatocytes isolated from male Wistar rats (incubation period: 24 h). In vitro, the test compounds were used at three concentrations (C1: low; C2: intermediate; C3: high, corresponding to the in vitro EC₂₀). 22 Genes were analyzed that either belong to the group of “stress response genes” or represent “proliferation associated genes”