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A recommended hepatocyte culture medium
| Supplement | Final concentration |
|---|---|
| Penicillin/streptomycin | 100 U/100 uM |
| l-glutamine | 1 % (0.292 g/l) |
| Hepes | 15 mM |
| FCS | 10° % |
| Hydrocortisone | 0.8 mg/l |
| Human insulin (100 U/ml) | 50 μU/ml (1.5 uM) |
| MEM non-essential amino acids | 1 % (0.1 mM) |
This medium is based on Williams’ E medium with the following supplements
