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Collagenase buffer composition for liver perfusion
| Reagents | Amount (ml) |
|---|---|
| Glucose solution (9 g/l) | 155 |
| Krebs Henseleit buffer pH 7.4 | 25 |
| HEPES pH 8.5 | 25 |
| Amino acid solution | 30 |
| CaCl2·2H2O solution (19 g/l) | 10 |
| Glutamine (7 g/l) | 2.5 |
| Collagenase | Add during perfusion with EGTA |
