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Suspension buffer composition for resuspension of primary hepatocytes
| Reagents | Amount (ml) |
|---|---|
| Glucose solution (9 g/l) | 124 |
| Krebs Henseleit Buffer pH 7.4 | 20 |
| HEPES pH 8.5 | 20 |
| Amino acid solution | 30 |
| CaCl2·2H2O solution (19 g/l) | 1.6 |
| Glutamine (7 g/l) | 2 |
| MgSO4·7H2O solution (24.6 g/l) | 0.8 |
| Bovine albumin fraction V | Add during perfusion with EGTA |
