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Calculation of the appropriate amount of gelled collagen to be used as a first layer
| Plate format | Collagen layer volume (uJ/well) | Amount of cells (×106) | Medium (ml/well) |
|---|---|---|---|
| Petri dish (∅ 6 cm) | 700 | 2 | 3 |
| 6-well plate | 350 | 0.85 | 2 |
| 12-well plate | 300 | 0.4 | 1 |
| 24-well plate | 100–200 | 0.2 | 0.5 |
