Table 2.
Summary of markers investigated
| Sample type | Target gene | Analytes (n) | Assay (manufacturer) |
|---|---|---|---|
| Primary | |||
| DNA | IL-6 | 10 SNPs | Bead-Chip arrays HumanHap550k V.3.0, OPA custom array (Illumina) |
| IL-6R | 18 SNPs | ||
| RNA | IL-6R | 3 probe sets | GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix) |
| Serum | IL-6 | 1 | Human IL-6 Immunoassay (Quantikine) |
| sIL-6R | 1 | Human sIL-6R Immunoassay (Quantikine) | |
| Secondary | |||
| DNA | IL-6 network genes | 233 SNPs (22 genes) | Bead-Chip arrays HumanHap550k V.3.0, OPA custom array (Illumina) |
| IL-6 pathway genes | 1004 SNPs (67 genes) | ||
| RNA | IL-6ST | 7 probe sets | GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix) |
For the DNA markers, SNPs in IL-6 and IL-6R are considered tier 1, IL-6 network genes are considered tier 2, and IL-6 pathway genes are considered tier 3.
IL-6, interleukin 6; IL-6R, IL-6 receptor; IL-6ST, IL-6 signal transducer; sIL-6R, soluble IL-6R; SNP, single-nucleotide polymorphism.