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Flow cytometric isolation of cancer stem cells based on ALDH activity. A: untreated control cells. B: Aldefluor-treated YES-2 cells in the absence of ALDH enzyme inhibitor DEAB. C: Aldefluor-treated YES-2 cells in the presence of DEAB. The red line shows the gate used to select for cells with high ALDH activity. SSC: side scatter.
