Fig 2.

Fluorescence intensity of ALDH1 reaction product in ESCC lines. A: Fluorescence in the ESCC lines, including YES-2CSC and YES-2S, after Aldefluor was applied to attached cells growing in culture. Scale bar = 100 µm B: Histograms showing the distribution of relative ALDH activity in the ESCC lines. C: Mean intensity of all ESCC lines. YES-2CSC is enriched in CSCs, and YES-2S is CSC-depleted. Oval indicates lines that are not significantly different from each other. Asterisks indicate the significantly different YES-2 lines. D: YES-2CSC compared with YES-2 to indicate the increase in stem-like cells in YES-2CSC. ADUs = Analog-to-digital units