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Time-course western blot validation of TGF-beta2 as a potential target of CD44 in MCF7F-B5 breast cancer cell line. Protein lysates were collected at different time points (18, 24 and 48hrs) following withdrawal of tet in the presence of hyaluronan (HA). The levels of expression of TGF-beta2 showed a time-dependent increase that was parallel to the increase of CD44 expression.
