Table 2. Set 2 and 2A of M-ARMS PCR a.
| Mutation | PCR product (bp) | Exon | Sequences (5' > 3') |
|---|---|---|---|
| W779G (c.2335T > G) | 294 (both primers) | 8 | F: TCGCTCATTGAACTCTCCTCCCT |
| R:ACCTTTGCCAAGTGTTCCAGACC (M)b | |||
| R: ACCTTTGCCAAGTGTTCCAGTCA (N)b | |||
| I1102T (c.3305T > C) | 415 (both primers) | 15 | F: GCAGTGCCAGGCTGTGCAAC (M) |
| F: GCAGTGCCAGGCTGTGCAAT (N) | |||
| R: CTCTGTAGCTTATGAGAAGCAAGACCG | |||
| c.3061-1G > A | 330 (both primers) | 14 | F: CAGTGAGTTGTGGTTGTTTTTGCCAA(M) |
| F: CAGTGAGTTGTGGTTGTTTTTGCCAG (N) | |||
| R: CTCTAAGTGGTTTTCCAGACCACACAG | |||
| N1270S (c.3809A > G) | 507 (both primers) | 18 | F: CCATGGTGGGGGATGGGGTAAG(M) |
| F: CCATGGTGGGGGATGGGGTAAA (N) | |||
| R: GTTTCAGGTCCTCTCCACAGTTTCTC | |||
| Lower control | 153 | F: AGTGGTCGTTTTAGCAGCAACAGAG | |
| R: GTGTTCATGTTACTGGGCCATCTCC | |||
| Upper control | 585 | F: CCACCGTCAGAGGAAGGAGAATTTC | |
| R:CTAGGTCAATGAAGAAGACCCTGTACAC |
aMutant-specific nucleotide is shown in bold and italic letter. Deliberate mismatch is underlined
bM, mutant-specific primers in Set 1; N, normal-specific primers in Set 1A