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. 2013 May 27;13(5):e8792. doi: 10.5812/hepatmon.8792

Figure 4. HBx Up-Regulated AKR1C1 Expression by Enhancing its Promoter Activity Through the -128/-88 Promoter Region.

Figure 4.

A) 5’-Serial deletion construct of the promoter region of the AKR1C1 gene were co-transfected with pCMV-Sport6-HBx and pRL-TK in HepG2 cells, the relative luciferase activity was determined. HBx+ means these groups have been transfected with pCMV-Sport6-HBx, HBx- means these groups have been transfected with pCMV-Sport6 (control). B) The same constructs described as Fig.4A were transfected with pRL-TK in HepG2.2.15 cells and the relative luciferase activity was measured. C) HepG2.2.15 cells were transfected with 0.6 μg of pAKR1C1-349/+59 or pAKR1C1-349/+59d-128/-88 and 0.2 μg pRL-TK, and relative luciferase activities were measured. D) HepG2.2.15 cells were transfected with 0.6 μg of pAKR1C1-641/+59 or pAKR1C1-641/+59d-349/-181 and 0.2 μg pRL-TK, and luciferase assay were performed. On the left side of graph is the schematic representation of the AKR1C1 reporter gene constructs, and on the right side, the bar graphs represent the relative levels of luciferase activity. Error bars indicate standard deviations (SD) obtained from three different experiments prepared in triplicate. *, P < 0.05; **, P < 0.01 as compared with control groups