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. 2013 Sep;142(3):275–288. doi: 10.1085/jgp.201310975

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© 2013 Perry et al.

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Figure 1. — Measurement of inactivation kinetics for WT and V535S Kv11.1 channels. (A) Voltage protocols used to measure the rates of onset of inactivation (i) and the rates of recovery from inactivation (ii), with key regions for the measurement of current highlighted in black. (B and C) Representative families of current traces measuring the rates of onset of inactivation (i) and rates of recovery from inactivation (ii) for WT (B) and V535S (C) Kv11.1 channels. Highlighted in bold are current traces at 0 mV (i) and −130 mV (ii) to aid comparison. (D) Chevron plots of the logarithm of the observed rates for onset of (open squares) and recovery from (closed squares) inactivation for WT (i) and V535S (ii) channels, plotted against voltage. Solid black lines are a fit of Eq. 1 (see Materials and methods), whereas the solid gray line in (ii) indicates k_obs,V for WT channels to aid comparison. Dashed lines indicate the derived unidirectional rate constants for the onset of (k_inact,V) and recovery from (k_rec,V) inactivation, with values at 0 mV indicated by arrows. The equilibrium constant for inactivation (K_eq) at 0 mV was calculated by: K_eq,0 = k_inact,0/k_rec,0 (Eq. 2 in Materials and methods).