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. 2013 Sep;142(3):275–288. doi: 10.1085/jgp.201310975

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© 2013 Perry et al.

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Figure 4. — Mutation of S4 residues affects inactivation at a midway point during the transition pathway. (A) REFER plots of the log forward unidirectional rate constant, log(k_inact,0), against the log equilibrium constant, log(K_eq,0), for inactivation, in families of mutations at individual residues: i, Leu529; ii, Leu530; iii, Leu532; iv, Val535. (B and C) REFER plots for all mutations at Leu529, Leu530, Leu532, and Val535 combined (B) or all S4 residue mutations (C). Data are presented as means ± SEM for 3–14 cells. The slope of the linear regression analysis (solid black lines) for each family of mutations represents the Φ-value, as indicated, and can be compared with the slope for all other S4 mutations (solid gray lines). ANCOVA was used to determine statistical significance; *, P < 0.05.