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. 2013 Jun 11;41(15):7302–7312. doi: 10.1093/nar/gkt411

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Saccharomyces cerevisae Rad51 and E. coli RecA are unable to disassemble nucleosomes. (A) Representative fluorescence emission spectra of 601 E-linker nucleosomes (12 nM) excited at 510 nM in the presence 130 mM KCl, ATP (250 µM) and 2 mM MgCl₂ (Mg²⁺) with S. cerevisae Rad51 (yRad51) at 0 (black), 200 (green), 500 (orange) and 1000 nM (purple). (B) Representative fluorescence emission spectra of 601D-linker nucleosomes (12 nM) excited at 510 nM in the presence 130 mM KCl, ATP (250 µM) and 2 mM MgCl₂ (Mg²⁺) with S. cerevisae Rad51 (yRad51) at 0 (black), 200 (green), 500 (orange) and 1000 nM (purple). (C) Representative fluorescence emission spectra of 601 E-linker nucleosomes (12 nM) excited at 510 nM in the presence 25 mM KCl, ATP (250 µM) and 2 mM Mg²⁺ with E. coli RecA (RecA) at 0 (black), 1 (purple), 2 (blue), 5 (red) and 10 µM (green). (D) Representative fluorescence emission spectra of 601D-linker nucleosomes (12 nM) excited at 510 nM in the presence 25 mM KCl, ATP (250 µM) and 2 mM MgCl₂ (Mg²⁺) with E. coli RecA (RecA) at 0 (black), 1 (purple), 2 (blue), 5 (red) and 10 µM (green). (E) Representative fluorescence emission spectra of 601 E-linker nucleosomes (12 nM) excited at 510 nM in the presence 130 mM KCl, ATP (250 µM) and 2 mM Mg²⁺ with E. coli RecA (RecA) at 0 (black), 1 (purple), 2 (blue), 5 (red) and 10 µM (green). (F) Representative fluorescence emission spectra of 601D-linker nucleosomes (12 nM) excited at 510 nM in the presence 130 mM KCl, ATP (250 µM) and 2 mM MgCl₂ (Mg²⁺) with E. coli RecA (RecA) at 0 (black), 1 (purple), 2 (blue), 5 (red) and 10 µM (green).