Table 2.
Synthetic interactions of Msl5 KH-QUA2 domain mutations
| MSL5 allele | Complementation |
||||
|---|---|---|---|---|---|
| mud1Δ | mud2Δ | nam8Δ | cbc2-Y24A | tgs1Δ | |
| WT | +++ | +++ | +++ | +++ | +++ |
| L169A | – | – | – | – | – |
| L176A | +++ | +++ | +++ | +++ | +++ |
| I189A | – | – | – | – | – |
Complementation of msl5Δ in the genetic backgrounds specified by otherwise functional MSL5 alleles was assayed by plasmid shuffle. L169A and I189A transformants failed to form FOA-resistant colonies at any temperature tested in each of the five strain backgrounds. By contrast, L176A supported growth on FOA and, by spot testing, +++ growth on YPD agar at all temperatures in all five strains.