Figure 3.

EcEσ⁷⁰ can use any NTP for initiation of efficient pRNA synthesis. pRNAs generated in vitro by EcEσ⁷⁰ from various RNAs or no RNA (indicated at top) when containing ³²P γ-GTP, ³²P γ-ATP or ³²P α-CTP (indicated by A, G or C label at top) were visualized on a denaturing gel. Lane M contains a 5′ end labeled oligonucleotide 19 nt in length for size comparison. These reactions were done at the same time, as the experiment shown in Figure 2B and therefore also have the smear from the ³²P γ-ATP preparation. The shorter product and larger abortive RNA that are enhanced from 6S(iGTP) RNA are indicated by asterisk and plus sign, respectively.