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. 2013 Jun 27;41(15):e152. doi: 10.1093/nar/gkt561

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Effect of RBMBs on RNA and protein expression. HT1080-GFP-96mer cells were microporated in the presence of RBMBs and placed in the incubator for 24 h. The concentration of RBMBs was varied from 0.4 to 4.8 μM. (A) The level of GFP RNA expression was then evaluated by RT-PCR. Control studies were performed on cells that were microporated in the absence of RBMBs (Ctr) or incubated with RBMBs, but not microporated (M-). (B) The level of GFP protein expression was evaluated by flow cytometry. Analagous studies were performed with CHO-GFP-96mer cells. (C) The effect of RBMBs on GFP RNA expression in CHO-GFP-96mer cells. (D) The effect of RBMBs on GFP protein expression in CHO-GFP-96mer cells. All studies were performed in triplicate. Samples were compared using a two-tailed unpaired t-test. An asterisk indicates statistically significant values of P < 0.05.