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Detection of single RNA transcripts with varying number of RBMB binding sites. RNAs containing (A) 0, (B) 2, (C) 4, (D) 8, (E) 16 or (F) 32 RBMB-binding sites were produced by in vitro transcription. The RNA transcripts were then hybridized with RBMBs and microporated into HT1080 cells. After 1–3 h, the cells were fixed and fluorescent images of the RBMB reporter signals were acquired. Representative images are shown.
