Fig. 5.

GRK5 mediates thymocyte apoptosis in polymicrobial sepsis: thymi from GRK5 WT and KO mice subjected to sham or CLP surgery were collected 20 and 36 h after surgery as described in Materials and Methods. Thymocytes were analyzed for total number (a), CD4+CD8+ (b), Annexin V+ and PI- staining and for caspase activity (c) as described in Materials and Methods (n = 5-6 per genotype for assessing total thymus cell number and Annexin V+ and PI- staining and n = 3-4 per genotype for the flow cytometry experiments). * p < 0.05, ** p < 0.01 compared to the corresponding septic WT groups.