Figure 6.
Expression of CD74-ROS in NSCLC cells induces invasion and E-Syt1 phosphorylation. A, cell lysates from the indicated NSCLC cell lines expressing CD74-ROS-WT or CD74-ROS-KM fusion proteins were immunoblotted for expression levels (9E10) and immunoprecipitated (IP) with an anti phosphotyrosine antibody and analyzed for the presence of E-Syt1 protein by immunoblotting (IB). B, graphical representation of in vitro invasion of NSCLC cell lines expressing CD74-ROS-WT or CD74-ROS-KM fusion proteins. The data is shown as fold increase in invasion when compared to empty pLXSN vector controls for the respective lines and is calculated using three independent clones for each construct.