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. 2013 Sep;5(9):a016766. doi: 10.1101/cshperspect.a016766

Figure 2.

Figure 2.

High-resolution imaging of ESCRT-III assemblies. (A) Electron micrograph of circular array protein scaffolds on membrane tubules in COS-7 cells expressing Snf7-1/CHMP4A and VPS4B(E235Q)-GFP. (Image from Hanson et al. 2008; adapted, with permission, from The Rockefeller University Press © 2008.) (B) Electron micrograph of reconstituted yeast Snf7 spirals assembled on lipid monolayers. (C) A helical polymer of reconstituted yeast Snf7, Vps24, and Vps2, imaged by electron microscopy. (Panels B and C are from Henne et al. 2012; adapted, with permission, from Cell Press © 2012.) (D) Electron tomography of high-pressure frozen cells, which show 17-nm filaments spiraling along the constriction zone. (This image is from Guizetti et al. 2011; adapted, with permission, from the author.) Scale bars, 100 nm, unless stated otherwise.