Figure 10.
Heightened CTL activity in rMVA-CD40L immunized mice. In order to characterize the lytic activity of B8- and OVA-specific CD8 T cells, an in vivo CTL assay was performed on day 56 after immunization. Unpulsed (CFSElow), B820–27-peptide pulsed (CFSEhigh) and OVA257–264-peptide pulsed (eFluor670high) splenic target cells were injected into rMVA and rMVA-CD40L immunized mice. Four hours post-transfer the ratio of unpulsed and peptide pulsed target cells was determined and the percent specific lysis calculated. Values represent mean ± SEM specific lysis of target cells of four mice per group. The experiment was performed twice with similar results.