Figure 11.

Improved virus control in B cell deficient mice. (A) In order to confirm that B cell deficient JHT mice used for challenge studies mount similar responses as C57BL/6 wt mice, JHT mice were immunized with rMVA and rMVA-CD40L. Seven days later the frequency of B8-specific CD8 T cells in the blood was determined by dextramer staining. Note that the percentage of B8-dextramer CD8 T cells after immunization is comparable between C57BL/6 wt mice and JHT mice (see Figure 6). Data are representative of five mice per group and two independent experiments. (B) JHT mice were immunized once with rMVA and rMVA-CD40L or twice with rMVA. Approximately 2 months after the first immunization, mice were infected with 1 × 10⁵ TCID₅₀ ECTV and monitored daily for disease symptoms and survival. Shown is the day of disease onset or death. Disease onset was defined as the appearance of symptoms such as skin lesions, swollen limbs, and tail or, necrotic skin areas. The data are compiled from three independent experiments. The numbers depict the number of mice that remained symptom-free of the total number of mice/group.