Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Aug 27;4:251. doi: 10.3389/fimmu.2013.00251

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Lauterbach, Pätzold, Kassub, Bathke, Brinkmann, Chaplin, Suter and Hochrein.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Transduction of FLDCs in vitro with rMVA-CD40L leads to superior DC activation and cytokine secretion. In vitro generated FLDCs were transduced with rMVA or rMVA-CD40L (MOI2). (A) Three, six, and nine hours after transduction DCs were analyzed for OVA (H-2Kb/OVA_257–264 complexes) and CD40L expression and the activation markers CD86 and CD80. Note that OVA expression is not different between rMVA and rMVA-CD40L. (B) At the same time points as in (A) supernatants were analyzed for IL-6, IFN-α, TNF-α, and IL-12p70 by a cytometric bead assay. Note that IL-12p70 and TNF-α were only detectable after transduction with rMVA-CD40L but not rMVA. Data are representative of at least two independent experiments. Shown is the mean of duplicate samples.