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. 2013 Aug 27;4:251. doi: 10.3389/fimmu.2013.00251

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Copyright © 2013 Lauterbach, Pätzold, Kassub, Bathke, Brinkmann, Chaplin, Suter and Hochrein.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increased CD8 T cell response after rMVA-CD40L immunization. C57BL/6 mice were immunized with rMVA and rMVA-CD40L on day 0 and 35. The primary and secondary B8- and OVA-specific CD8 T cell response was quantified by dextramer staining of PBL at the indicated time points as shown in Figure 1A. (A) The kinetic analysis shows the mean percentage ± SEM of CD8⁺ dextramer⁺ T cells among PBL. (B) B8- and OVA-specific CD8 T cells were also analyzed flow cytometrically for surface expression of CD127. Data are represented as the mean ± SEM GMFI. Data are representative of five mice per group and two independent experiments.