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. 2013 Jun 14;24(4):228–240. doi: 10.1089/hgtb.2012.190

FIG. 1.

FIG. 1.

Strategy of RD-MolPack development. (a) Schemes of plasmids and vectors used in this study. pPolh, polyhedrin promoter; attB1, attachment B1; ITR, inverted terminal repeat; CMV, cytomegalovirus promoter; In, intron; RRE, rev responsive element; A, polyA sequence; IRES, internal ribosome entry site; SD, splice donor; SA, splice acceptor; Ψ, packaging signal; WPRE, woodchuck hepatitis post-transcriptional regulatory element; cPPT, central polypurine tract; hPGK, human phosphoglycerate kinase promoter. (b) Flow chart of the development strategy of PK-7 and RD-MolPack-Chim3 packaging cells. (c) Cartoon of the Rep78-mediated integration of the AAV-GPR vector into HEK-293T host genome. AAV Rep78 promotes the excision of the ITR-flanked AAV-GPR cassette and facilitates the integration into human chromosomes in either single or concatameric configuration.