Tenofovir prevents autotransduction in RD2-MolPack-Chim3 clones. Quantitative PCR analysis of PΔN-Chim3 VCN in two RD2-MolPack-Chim3 clones, the RD2-MolPack-Chim3.61 clone, carrying 3.5 copies (a), and the RD2-MolPack-Chim3.2 clone, carrying 20 copies (b), and in several subclones obtained either in the presence or absence of tenofovir. (c) Southern blot analysis of gDNA of the representative RD2-MolPack-Chim3.61clone, grown either in the presence or in the absence of tenofovir, and digested with SacI to monitor vector integrity over time at three different time points as indicated.