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. Author manuscript; available in PMC: 2013 Aug 27.
Published in final edited form as: Methods Mol Biol. 2009;534:147–156. doi: 10.1007/978-1-59745-022-5_11

Table 1.

GAG-depolymerizing enzymes

Enzyme class Enzyme Specificity
Heparinase I HNx6x(α1,4)-IdoA2S
II HNy6x(α1,4)-U2x
III HNy6x(α1,4)-GlcA & HNAc(α1,4)-IdoA
Chondroitinase AC GalNAc4x6x(β1,4)-GlcA2x
B -GalNAc4x6x(β1,4)-IdoA2x-
ABC I -GalNAc4x6x(β1,4)-U2x-
ABC II GalNAc4x6x(β1,4)-U2x-
C -GalNAc6x(β1,4)-GlcA-
Keratanase I HNAc6x-Gal(β1,4)-HNAc6x-
II HNAc6S(β1,3)-Gal6x-HNAc6S
Hyaluronate Lyase -HNAc(β1,4)-GlcA-

x Sulfated or unsubstituted; S Sulfated; y Acetylated or sulfated; Ac Acetylated; Gal Galactose; H Hexosamine; U Uronic acid of either the GlcA or IdoA epimer state. Heparinases I, II, and III are from Pedobacter heparinus. Chondroitinases AC, B, and C are also from Pedobacter heparinus. Hyaluronate lyases are available from a variety of sources including Peptostreptococcus, Staphylococcus, Streptococcus, and Apis mellifera, among others. Keratanases mediate the depolymerization of keratan sulfate and are not lyases, but rather hydrolases