Table 3.
Oligonucleotides used
| Gene or region and primer designation | Primer sequence (5′–3′) |
|---|---|
| 18S rRNA gene | |
| 501 | CATGGCCGTTCTTAGTTGGT |
| 301 | ATTGCCTCAAACTTCCATCG |
| ACT1 | |
| 501 | GCCTTCTACGTTTCCATCCA |
| 301 | GGCCAAATCGATTCTCAAAA |
| ASR1 | |
| 501 | CTTAGTAAGTGAGAAATATGGCAAGTAAT |
| 301 | CTATATTTGAGTTTGTTCAACTGTTTTCTAT |
| CIT2 (promoter region) | |
| 502 | AAAAGGGCCCATTATTCTCG |
| 303 | CTTAATAATGAGGAACGAACACCA |
| CIT2 (ORF) | |
| 501 | AGATGGCAAAATGGGTGAAG |
| 301 | GATTAGCACGCCCATGAAGT |
| Intergenic region | |
| IntergChrV-F | TGTTCCTTTAAGAGGTGATGGTGAT |
| IntergChrV-R | TGTTCCTTTAAGAGGTGATGGTGAT |
| MFα2 | |
| 501 | CTACCGCCAGTGGGCTATT |
| 301 | CTACCGCCAGTGGGCTATT |
| PMA1 (promoter region) | |
| P-503 | AAAGGCCAAATATTGTATTATTTTCAA |
| P-302 | TTGGTGTTATAGGAAAGAAAGAGAAAA |
| PMA1 (ORF) | |
| 6(forw) | ATATTGTTACTGTCGTCCGTGTCTGGAT |
| 6(rev) | ATTAGGTTTCCTTTTCGTGTTGAGTAGA |
| PYK1 (promoter region) | |
| P-502 | ACAAGACACCAATCAAAACAAA |
| P-301 | AGTCAGAACCAGCAACAACG |
| PYK1 (ORF) | |
| 4(forw) | CTATGGCTGAAACCGCTGTCATTG |
| 4(rev) | CAGCTCTTGGGCATCTGGTAAC |
| RPB1 | |
| 507 | GATGCTGACAACGACACTGAAG |
| 310 | CGCAAGCCATGATTACTGG |
| RPL3 | |
| 501 | AAGAAGGCTCATTTGGCTGA |
| 301 | AACACCTTCGAAACCGTGAC |
| RPS2 | |
| 501 | TGGTAAGTGTGGTTCCGTCA |
| 301 | AATGGAGAAACTGGCAATGG |
| SPP2 | |
| 501 | TGCTTTACTTCGGGGTATGG |
| 301 | TGAAGCCTCCTCCACGTTTA |
| SRB10 | |
| 501 | AAGGGTATACTGAAGTTAGTAATTTTGCTTC |
| 301 | AAAAAGTTTAATGAATATAATAGTGACAGTGCT |