Fig 1.

mTORC1 suppresses ATGL promoter activity. Wild-type (WT) and TSC2^−/− MEFs were transiently transfected with different lengths of mouse ATGL luciferase promoter constructs, as indicated, together with eGFP. After 48 h, the cells were washed three times in cold PBS and harvested in the reporter lysis buffer. The absolute luciferase activity (A) and the relative luciferase activity (B) in cell lysates was assayed as described in Materials and Methods and normalized based on the eGFP fluorescence. Experiments were repeated four times, and data are presented for triplicate samples as means ± the standard deviations (SD). **, P < 0.01.