Fig 3.

Msx1 interacts with tooth-specific transcription factors in vivo. pCMV-FLAG-Msx1 or control vector (pCMV-FLAG-Tag2B) was coexpressed with transcription factors Dlx2 (A), Lhx6(B), Snail (C), Lhx8 (D), Sp3 (E), and Lef1 (F) in C3H10T1/2 cells. After 36 h, cell lysate (20%) of each was taken as the input sample to verify the level of expression (first and second lanes). All the transcription factors tested were detected only in the presence of FLAG-Msx1 in the immunoprecipitation samples (fourth lanes) and not in the control samples (third lanes). IP, immunoprecipitation; IB, immunoblotting.