Fig 4.
Hif-p4h-2gt/gt hearts have larger capillaries. (A) Resin casts of heart vasculature. The LAD and RCA are indicated by dotted lines. (B and C) Baseline α-SMA (red) and isolectin (green) costaining of hearts and quantification of the number (wild-type [wt] hearts, n = 5; Hif-p4h-2gt/gt hearts, n = 4) and size (wild-type hearts, n = 6; Hif-p4h-2gt/gt hearts, n = 6) of collateral vessels. (D and E) Baseline Pecam-1-stained hearts and quantification of the number and size of capillaries (wild-type hearts, n = 3; Hif-p4h-2gt/gt hearts, n = 3). (F) TEM of wild-type and Hif-p4h-2gt/gt hearts. Large capillaries (c) are visible in the latter. (G) Quantification of the number of nuclei per capillary on the basis of TEM analysis (wild-type hearts, n = 3; Hif-p4h-2gt/gt hearts, n = 3).