Fig 4.

B4.5 and C11.12 confer Polycomb-mediated repression from integrated genomic sites. (A) Schematic of plasmids used for genomic integration. B4.5(+) and C11.12(+) denote constructs containing the repressive elements. B4.5(−) and C11.12(−) are the integrated constructs that no longer bear the repressive elements. (B) Luciferase activity of the integrated B4.5 and C11.12 elements before and after FLP recombinase activity. (C and D) ChIP-qPCR for BMI1, SUZ12, YY1, H3, and H3K27me3 from the integrated constructs. The PCR primers target the promoter, luciferase gene, and endogenous control regions relevant to either B4.5 or C11.12. The H3K27me3/H3 graph calculates the enrichment of H3K27me3 relative to the abundance of H3 present at the sites. Data are represented as the mean (n = 3) ± SEM.