Fig 1.

Comparative expression analysis of female wild-type and Smchd1^−/− embryos at E10.5. (A) Total RNAs from three female wild-type and three Smchd1^−/− embryos were processed individually and hybridized to Affymetrix mouse genome 430 2.0 arrays. A total of 215 probe sets were found to be differentially expressed following data processing (FDR, ≤30%), with 154 up- and 61 downregulated probe sets in Smchd1^−/− embryos showing a log₂ fold expression change ranging from −3 to +3 and overlapping X-linked and autosomal genes. (B) Scatter plot of normalized expression values (in log₂ scale) of 569 X-linked genes in wild-type versus mutant embryos. Three individual genes upregulated in mutant embryos are highlighted in color. (C) Scatter plot of normalized expression values (in log₂ scale) of 14,962 autosomal genes in wild-type versus mutant embryos. A number of individual genes that are up- or downregulated in mutant embryos are highlighted in color.