Fig 3.
Comparison of rad53-4AQ cells with rad53-K227A and dun1Δ cells. (A and B) Tetrad dissection of diploid strains heterozygous for rad9Δ, sml1Δ, and either dun1Δ or rad53-K227A. Colonies originating from the same tetrad are in columns. Indicated genotypes are marked with circles or squares. (C) Western blot analysis comparing WT, rad53-4AQ, rad53-K227A, and dun1Δ cells at the indicated time points after release from G1 arrest. All four strains were processed at the same time, and the same WT + MMS reference sample was used for both gels.