Fig 7.

Essential functions of Rad53 during normal S phase. (A) Summary of dNTP pools, kinase activity and viability of the indicated yeast mutants. “*” denotes phenotypes that were deduced from other experimental evidence or implied from other mutant strains. For example, low dNTP pools for the rad53-D339A strain were inferred from its inability to activate Dun1 (see Fig. 6), and very low Rad53 kinase activity in the rad53-4AQ-K227A strain was implied by rad53-K227A data (see Fig. 4C to F and Fig. 6). (B) Schematic diagram of Rad53 activation during unperturbed S phase in WT and rad53-4AQ cells. Numbers indicate the order of events, and dashed lines indicate defects in the mutant strain.