Fig 6.
The Ikaros Δexon 6 isoform does not interact with Cdk9. (A) Schematic view of Ik1 and the Ik1Δex6 isoform. (B and C) Total cell lysates of COS-7 cells transfected (Tr) with expression vectors encoding Ik1-FH (Ik1), FH-Ik1Δex6 (Ik1Δex6), GATA1 (G1), GATA2 (G2), or GATA3 (G3) were immunoprecipitated with Flag, Cdk9, GATA1, GATA2, or GATA3 antibodies. Co-IP samples were analyzed by Western blotting with HA, Cdk9, Mi2, GATA1, GATA2, or GATA3 antibodies. •, nonspecific band. (D) Confocal IF of COS-7 cells expressing Ik1-FH (Ik1) or FH-Ik1Δex6 (Ik1Δex6); Ik was detected with mouse anti-HA and FITC-conjugated anti-mouse antibodies. Results with representative COS-7 cells are shown.