Fig 5.

Colocalization of 14-3-3ζ with Btk. Cos-7 cells were cotransfected with GFP-Btk-wt and RFP–14-3-3ζ plasmids in the presence or absence of siRNA–14-3-3ζ. Forty-eight hours posttransfection, the cells were fixed and stained with DAPI (4′,6-diamidino-2-phenylindole). GFP-Btk is shown in green, while RFP–14-3-3ζ is shown in red. The merged images indicate colocalization of Btk and 14-3-3ζ in membrane ruffles and in the perinuclear region (yellow). The data are representative of at least 3 different experiments.