Table 1.
Unique peptide sequences confirmed by MS-MS analysisa
| No. of MS-MS spectra | No. of unique peptides | Mascot score | Coverage (%) | Peptide sequence |
|---|---|---|---|---|
| 5 | 4 | 182 | 19 | SVTEQGAELSNEER |
| EKIETELR | ||||
| DICNDVLSLLEK | ||||
| YLAEVAAGDDKK | ||||
| 3 | 2 | 170 | 13 | SVTEQGAELSNEER |
| TAFDEAIAELDTLSEESYK | ||||
| 3 | 2 | 133 | 11 | SVTEQGAELSNEER |
| YLAEVAAGDDKK | ||||
| 4 | 2 | 124 | 9 | LAEQAER |
| SVTEQGAELSNEER | ||||
| 3 | 2 | 123 | 11 | SVTEQGAELSNEER |
| DICNDVLSLLEK | ||||
| 3 | 2 | 117 | 11 | SVTEQGAELSNEER |
| YLAEVAAGDDKK | ||||
| 4 | 3 | 108 | 10 | SVTEQGAELSNEER |
| FLIPNASQAESK | ||||
| NLLSVAYK | ||||
| 2 | 2 | 101 | 11 | SVTEQGAELSNEER |
| YLAEVAAGDDKK |
a
The selections were based on high Mascot scores. The overall coverage of all amino acid sequences (92 amino acids [aa]) of unique peptides was calculated on primary sequences of the 14-3-3ζ isoform (245 aa) to be 37.5% (92 aa out of 245 aa). Proteomics analyses were based on 5 independent experiments, and each individual MS-MS data set was collected from 15 samples.