Table 2.

Distribution of expressed proteins in nuclear, cytoplasmic, membrane, and perinuclear regions^a

Protein(s) expressed	% of cells expressing protein(s)b						% of cells with cytoplasmic protein(s) showing membrane or perinuclear localization
	GFP-Btk or GFP-Btk-NLS			DsRed–14-3-3ζ or DsRed-Liar			Membrane		Perinuclear
	N Only	C Only	N + C	N Only	C Only	N + C	Btk	14-3-3ζ	Btk	14-3-3ζ
GFP-Btk	0	91	9				82		13
DsRed–14-3-3ζ				0	92	8		4		86
GFP-Btk-NLS	100	0	0
GFP-Btk + DsRed–14-3-3ζ	0	94	6	0	95	5	58	26	41	71
GFP-Btk-NLS + DsRed–14-3-3ζ	12	0	88	0	78	22
GFP-Btk + DsRed-Liar	0	100	0	19	0	81
GFP-Btk + siRNA–14-3-3ζ	1	70	29
GFP-Btk + DsRed-Liar + siRNA–14-3-3ζ	0	100	0	11	0	89

^aMean values were calculated for 100 cells from 3 independent experiments.

^bN, nuclear; C, cytoplasmic.