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. 2013 Sep;19(9):1192–1199. doi: 10.1261/rna.039503.113

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© 2013; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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FIGURE 4. — Analysis of kinetic isotope effect. (A) Kinetics of m¹G37-tRNA synthesis catalyzed by MjTrm5, showing a virtually identical k_obs of 0.11 sec⁻¹ in H₂O and in D₂O, with standard errors of 0.02. (B) Kinetics of m¹G37-tRNA synthesis catalyzed by HsTrm5, showing a virtually identical k_obs of 0.012 sec⁻¹ with standard errors of 0.001. Data for H₂O are shown in closed triangles; data for D₂O are shown in closed circles.