Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep;19(9):1208–1217. doi: 10.1261/rna.039446.113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

PMC Copyright notice

FIGURE 2. — Deletion of ASC1 or HEL2, but not LTN1, results in improved expression of genes downstream from CGA codons at amino acid 318. (A,B) Renilla (A) and firefly (B) luciferase activity of the Renilla-firefly luciferase fusion reporter constructs containing either no insert or the indicated RGS-(His)₆-(Arg)₄ insertions in the wild-type and ltn1-Δ strains. Strains were transformed with low copy plasmids with or without the LTN1 gene as indicated. Schematic of the reporter is included above panel A. (C,D) Mean GFP/RFP fluorescence (arbitrary units) of the Renilla-Arg₄-GFP fusion (schematic) reporter in wild-type, hel2-Δ, or asc1-Δ strains. Two independent asc1-Δ mutant strains were constructed, confirmed by genomic PCR, and tested here; they are designated Δ1, Δ2. A diagram of the reporter is shown above panel C.